A methanol/dioxygen biofuel cell that uses NAD+-dependent dehydrogenases as catalysts: application of an electro-enzymatic method to regenerate nicotinamide adenine dinucleotide at low overpotentials
نویسندگان
چکیده
Diaphorase catalyzes efficiently the oxidation of NADH to NAD+ by benzylviologen. This reaction was used to regenerate the NADf required for the biocatalyzed oxidation of methanol to CO, using NADf-dependent dehydrogenases. A rnethanol/dioxygen biofuel cell was assembled using these bioreactions in the anode compartment. The open-circuit voltage of the cell was 0.8 V, and the maximum power output of the cell was 0.67 mw/cm2 of graphite at 0.49 V. These results reflect the low overpotential at which NADH is oxidized and demonstrate a new approach to lowering voltage losses in biofuel cells due to activation overpotentials.
منابع مشابه
Hydrogen generation from methanol at near-room temperature† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01778b Click here for additional data file.
As a promising hydrogen storage medium methanol has many advantages such as a high hydrogen content (12.5 wt%) and low-cost. However, conventional methanol-water reforming methods usually require a high temperature (>200 °C). In this research, we successfully designed an effective strategy to fully convert methanol to hydrogen for at least 1900 min (∼32 h) at near-room temperature. The strategy...
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